reference mabs atcc 19977 strain Search Results


99
ATCC electrocompetent atcc 19977
Electrocompetent Atcc 19977, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
ATCC m abscessus subsp abscessus atcc 19977 mabs
RNS-dependent activity of GSK1867513A against nontuberculous mycobacteria M. avium and M. <t>abscessus</t> . (A) Concentration-dependent mycobactericidal activity of GSK513A against M. avium subspecies hominissuis MAC101 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in one experiment. (B) Concentration-dependent mycobacteristatic activity of GSK513A against M. abscessus subspecies abscessus <t>ATCC</t> <t>19977</t> in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in two experiments.
M Abscessus Subsp Abscessus Atcc 19977 Mabs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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90
MiddleBrook Pharmaceuticals 10 6 cfu/ml mab 19977
RNS-dependent activity of GSK1867513A against nontuberculous mycobacteria M. avium and M. <t>abscessus</t> . (A) Concentration-dependent mycobactericidal activity of GSK513A against M. avium subspecies hominissuis MAC101 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in one experiment. (B) Concentration-dependent mycobacteristatic activity of GSK513A against M. abscessus subspecies abscessus <t>ATCC</t> <t>19977</t> in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in two experiments.
10 6 Cfu/Ml Mab 19977, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Novogene illumina pe150 platform
RNS-dependent activity of GSK1867513A against nontuberculous mycobacteria M. avium and M. <t>abscessus</t> . (A) Concentration-dependent mycobactericidal activity of GSK513A against M. avium subspecies hominissuis MAC101 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in one experiment. (B) Concentration-dependent mycobacteristatic activity of GSK513A against M. abscessus subspecies abscessus <t>ATCC</t> <t>19977</t> in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in two experiments.
Illumina Pe150 Platform, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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96
ATCC mab 062600 1635 1 6 50 50 50 50 mab 030804 1651 1 6 50 50 50 50 mab 010708 1655 1 6 50 50 50 50 open
RNS-dependent activity of GSK1867513A against nontuberculous mycobacteria M. avium and M. <t>abscessus</t> . (A) Concentration-dependent mycobactericidal activity of GSK513A against M. avium subspecies hominissuis MAC101 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in one experiment. (B) Concentration-dependent mycobacteristatic activity of GSK513A against M. abscessus subspecies abscessus <t>ATCC</t> <t>19977</t> in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in two experiments.
Mab 062600 1635 1 6 50 50 50 50 Mab 030804 1651 1 6 50 50 50 50 Mab 010708 1655 1 6 50 50 50 50 Open, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Becton Dickinson middlebrook 7h9
RNS-dependent activity of GSK1867513A against nontuberculous mycobacteria M. avium and M. <t>abscessus</t> . (A) Concentration-dependent mycobactericidal activity of GSK513A against M. avium subspecies hominissuis MAC101 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in one experiment. (B) Concentration-dependent mycobacteristatic activity of GSK513A against M. abscessus subspecies abscessus <t>ATCC</t> <t>19977</t> in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in two experiments.
Middlebrook 7h9, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mab  (ATCC)
93
ATCC mab
NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin <t>(red).</t> <t>MAC</t> and <t>MAB:</t> GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.
Mab, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
ATCC b1mcf9 b1mcf9 myca9 bacteria mab 2851c mycobacterium abscessus
NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin <t>(red).</t> <t>MAC</t> and <t>MAB:</t> GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.
B1mcf9 B1mcf9 Myca9 Bacteria Mab 2851c Mycobacterium Abscessus, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


RNS-dependent activity of GSK1867513A against nontuberculous mycobacteria M. avium and M. abscessus . (A) Concentration-dependent mycobactericidal activity of GSK513A against M. avium subspecies hominissuis MAC101 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in one experiment. (B) Concentration-dependent mycobacteristatic activity of GSK513A against M. abscessus subspecies abscessus ATCC 19977 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in two experiments.

Journal: ACS Infectious Diseases

Article Title: Diaryl-Aminoindazole with Extensive In Vitro Mycobactericidal Activity Dependent on Exposure to Reactive Nitrogen Species

doi: 10.1021/acsinfecdis.5c00879

Figure Lengend Snippet: RNS-dependent activity of GSK1867513A against nontuberculous mycobacteria M. avium and M. abscessus . (A) Concentration-dependent mycobactericidal activity of GSK513A against M. avium subspecies hominissuis MAC101 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in one experiment. (B) Concentration-dependent mycobacteristatic activity of GSK513A against M. abscessus subspecies abscessus ATCC 19977 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in two experiments.

Article Snippet: Mtb H37Rv, M. abscessus subsp. abscessus ATCC 19977 (Mabs) and M. avium subsp. hominissuis MAC101 (MAC) were cultured in 7H9 medium with 0.2% glycerol, 0.02% tyloxapol and 10% OADC (Difco) (complete 7H9).

Techniques: Activity Assay, Concentration Assay

NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin (red). MAC and MAB: GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Transcriptional Response of Respiratory Epithelium to Nontuberculous Mycobacteria

doi: 10.1165/rcmb.2017-0218OC

Figure Lengend Snippet: NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin (red). MAC and MAB: GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.

Article Snippet: Strains of MAC (ATCC 35717) and MAB (ATCC 19977) were used.

Techniques: Infection, Labeling, Confocal Microscopy, Immunostaining, Bacteria

The percentage of ciliated cells decreased in NTM-infected respiratory epithelium. (A) Representative images of NHBE cells derived from a 67-year-old woman (67F). Infected or uninfected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin (red). MAC and MAB: GFP (green). Scale bars: 100 μm. (B) Tiled images of infected or uninfected respiratory epithelium were analyzed and the percentage of ciliated cells was determined as the total number of cells high in β-tubulin staining. Data are expressed as the mean ± SEM (three different donors [67F, 44F, and 67M], two fields of view per each donor). *Significant difference between infected and uninfected epithelium at Days 1 and 3 after infection (P < 0.05, one-way ANOVA followed by post hoc tests).

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Transcriptional Response of Respiratory Epithelium to Nontuberculous Mycobacteria

doi: 10.1165/rcmb.2017-0218OC

Figure Lengend Snippet: The percentage of ciliated cells decreased in NTM-infected respiratory epithelium. (A) Representative images of NHBE cells derived from a 67-year-old woman (67F). Infected or uninfected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin (red). MAC and MAB: GFP (green). Scale bars: 100 μm. (B) Tiled images of infected or uninfected respiratory epithelium were analyzed and the percentage of ciliated cells was determined as the total number of cells high in β-tubulin staining. Data are expressed as the mean ± SEM (three different donors [67F, 44F, and 67M], two fields of view per each donor). *Significant difference between infected and uninfected epithelium at Days 1 and 3 after infection (P < 0.05, one-way ANOVA followed by post hoc tests).

Article Snippet: Strains of MAC (ATCC 35717) and MAB (ATCC 19977) were used.

Techniques: Infection, Derivative Assay, Labeling, Confocal Microscopy, Immunostaining, Staining